Ajuga Orientalis L Anatomy Studies

Ajuga Orientalis L Anatomy Studies The anatomical studies of medicinal taxon Ajuga orientalis L. (Lamiaceae) from Turkey Abstract. Ajuga orientalis[a1], with a wide distribution area in Turkey, is traditionally used medicine in the treatment of some skin diseases in Anatolia. The aim of this study is to determine the anatomical characteristics of the root[a2], leaf, petiole, calyx and corolla of medicinal taxon [a3]A. orientalis in cross sections. As a result of the study, it was found that the pith rays of root are composed 3-4 rowed cells and stem was quadrangular[a4]. There were glandular and non-glandular hairs on the surface layers on stem, leaves, petiole, calyx and corolla. Starch particles were also identified[a5] in the cortex cells of stem. The stomata were diastic[a6] and the leaf was bifacial. There were one big vascular bundle in the center and 4-5 small vascular bundles on each corner of the petiole. It was also determined that adaxial epidermis cell shapes of corolla are papillose type. Key words: Ajuga orientalis, anatomy, medicinal plant, Turkey INTRODUCTION The Lamiaceae is a large family showing natural distribution. Most of species belonging to this family are shrubby and herbaceus, and trees are extremely rare (Heywood, 1978). The family with its more than 250 genera and approximately 7000 species, has a cosmopolitan distribution (Thorne, 1992). According to BaÃ…Å ¸er (1993), Turkey is accepted as a gene center for this family. Many species of this family are aromatic and are often used as herb spices, folk medicines and fragrances (Werker et al., 1985). With their pleasant fragrance, many species of Lamiaceae have been used as herbal teas in Turkey. Many of species are used as raw material in the cosmetic industry. Some species are traditionally used as medicinal plants (Baytop, 1984). It was reported that some Ajuga L. and Salvia L. species are cultivated as ornamental plants (Baytop, 1984; Ãâ€"zdemir and Ã…Å ¾enel, 2001; Akà §in et al., 2006). In addition to this, Lamiaceae has great importance due to its economical valu e and its variety of species. The genus Ajuga L. belongs to Lamiaceae family. Ajuga L. is represented in Turkey by 13 species and 22 taxa, six species and one subspecies being endemic (Davis et al., 1982-1988). Ajuga species are used in folk medicine in different parts of the world for the treatment of rheumatism, gout, asthma, diabetes, malaria, ulcers and diarrhea and have antibacterial, antitumor, antifeedant, and vulnerary properties (Chen et al., 1996; Ben Jannet et al., 2000). Baytop (1999) reported that some Ajuga species generally known as â€Å"mayasÄ ±l otu† in Turkey have been widely used for their aromatic, diuretic, antipyretic, tonic, diaphoretic, astringent, bitter and homeopathic properties in the Turkish folk medicine. Ajuga orientalis L. one of the species of Ajuga genus is used against some skin diseases by hug on the skin in Anatolia (Koyuncu et al., 2010). Although many species of Lamiaceae family are investigated anatomically (ÇobanoÄÅ ¸lu, 1988; Uysal et al., 1991; Ãâ€"zdemir and Altan, 2005; AktaÃ…Å ¸ et al., 2009) there is no anatomical study of A. orientalis L. in literature. Due to its medicinal importance mentioned above in this study, the purpose of this study is to determine the anatomical characteristics of A. orientalis L. MATERIALS AND MEDHODS In the present study, the plant specimens of A. orientalis were collected during the flowering period and natural populations in A5 Amasya (in the vicinity of Direkli village, open areas, at 1800 m, June 2012, Ä °Ãƒâ€"ztà ¼rk ÇalÄ ± 461) which is a city in the Black Sea region of Turkey. Its taxonomical description was carried out according to Davis (1982). Anatomical investigations were performed using an average of fresh specimens kept in 70% alcohol. Cross sections of root, stem, laeve, petiole, calyx and corolla were taken from 30 specimens of A. orientalis and 50 measurements were conducted for each parameter. Transverse sections were made by hand using commercial razor blades and stained with Sartur reactive (ÇelebioÄÅ ¸lu and Baytop, 1949). Measurements in the sections were performed under a Leica ICC50 HD binocular light microscope by using a Leica Digital Camera and objectives used were x10 and x40. Photographs were taken with a Leica ICC50 HD binocular light microscope and a Leica Digital Camera. RESULTS In cross-sections taken from the root, stem, leaf, petiole, calyx and corolla of A. orientalis , the following significant properties were observed below. In the transverse section of the root, there was a periderm which was the outermost layer of the root of A. orientalis (Figure 1). The periderm had 8-9 layers. The dimensions of periderm cells were 15 – 60 X 15 – 57.5  µm (Table 1). Beneath the periderm, there was the multi-layered cortex, composed of ovaidal and parancyhmatic cells. The cortex was 9-10 layers. The cambium, composed of 3-4 layered, was located between the xylem and the phloem. Beneath the cambium, there were xylem tissue (7.5 – 15 X 7.5 – 20  µm) composed of regular trachea and tracheid cells. Xylem cells were also present in the center, so the pith is not seen in the center. There were 3-4 layered primary pith rays between the secondary xylem cells. A. orientalis, as a typical characteristic of the Lamiaceae, had a 4-angle stem. Its epidermis usually had one layer composed of ovoidal cells (12.5 – 42.5 X 17.5 – 50  µm). The upper surface was covered with a cuticle (1.25 – 2.5  µm). There were glandular and non-glandular hairs on the epidermis (Figure 2). Transverse section of the stem revealed 9-10 layered collenchyma placed on the corners. The cortex was composed of 6-7 layered paranchymatous cell. The dimensions of paranchymatous cells were 27.5 – 75 X 15 – 55  µm (Table 1). Starch particles were also observed in the paranchymatous cells of stem (Figure 3). There were 2-3 layers of sclerenchyma in the outer side of phloem. Right beneath the phloem, there was a 1-2 rowed cambium layer. The xylem tissue existing beneath the cambium was composed of regular trachea and tracheid cells. The vascular bundles were bigger on the corner than other parts of stem. Between the corners there were als o numerous small bundles in the A. orientalis stem. The vascular bundles were collateral (Figure 2). The pith was wide and consist of flat cells with intercellular spaces. There was a cavity in the centre of the pith. In the leaves, the epidermis was single layered on upper and lower surface. There were glandular and non-glandular hairs on epidermis. The cuticle was 2.5 – 5  µm thick. Just beneath the upper epidermis cells, there were 2-3 rowed palisade parenchyma cells (Figure 5). The dimensions of the palisade parenchyma cells were 12.5 – 25 X 25 – 50  µm (Table 1). The 2-3 rowed spongy parenchyma existed beneath the palisade. The spongy parenchyma covered less space than does the palisade parenchyma. The collateral vascular bundle was located in the midrib region (Figure 5). There were sclerenchyma cells in the outer side of phloem. All vascular bundles in the leaves were surrounded by bundle sheet cells. The stoma was diastic and the leaf was bifacial (Figure 4 and Figure 5). The stoma presented on upper and lower surfaces of the leaf (Figure 4). Both adaxial and abaxial epidermis cells were single layered in the petiole (Figure 6). The dimensions of the adaxial epidermis cell were 15 – 45 X 17.5 – 27.5  µm while those of the abaxial epidermis one were12.5 – 27.5 X 15 – 17.5  µm (Table 1). There were a lot of glandular and non-glandular hairs on epidermal cells which were ovoidal-rectangular shapes. Parenchymatic cortex cells were 10-11 layered. There was one big vascular bundle in the center and 4-5 small vascular bundles at each corners of petiole. The vascular bundles were surrounded by sclerenchymatic cells (Figure 6). There were parenchmatic bundle sheets on the all vascular bundles. The type of vascular bundle was collateral (Figure 6). There were 2-3 layered collenchyma in the area between the corners. In the calyx, the adaxial epidermis cells were smaller than abaxial epidermis cells. The dimensions of adaxial cuticle were 1.25 – 2.5  µm whereas the those of the abaxial cuticle is 2.5 – 3.75  µm (Table 1). Parenchymatic cells were flat ovoidal. There were glandular and non-glandular hairs on the epidermis (Figure 7a). In the cross-section of corolla, cuticle was present on both abaxial and adaxial epidermis cells covered by glandular and non-glandular hairs. The shapes of adaxial epidermis cells were papillose type (Figure 8). Beneath the adaxial epidermis, there were the parenchyma cells with intercellular spaces (Figure 7b). There was a vascular bundle in the midrib. DISCUSSION The present study provided useful information on the anatomy of A. orientalis. Few studies on the species A. orientalis had been found in literature (Sajjadi and Ghannadi, 2004; Koyuncu et al., 2010). But, the measurements and observation of anatomical characters belonging to the medicinal taxon A. orientalis were reported for the first time in the present paper. Metcalfe and Chalk (1972) determined some imported anatomical information about root anatomy of Lamiaceae family. They stated that the pith rays of roots of the family are 2-12 or more rowed cells. It was found that the pith rays of A. orientalis were composed 3-4 rowed cells. These findings were consistent with those of Metcalfe and Chalk (1972) and those of some studied species of Lamiaceae (Baran and Ãâ€"zdemir, 2006; Ãâ€"zkan and Soy, 2007; Baran and Ãâ€"zdemir, 2009). Metcalfe and Chalk (1972) also stated that the members of Lamiaceae family have quadrangular with well-defined collenchyma in the four angles and scleranchymatous tissue surrounds the phloem groups of vascular bundles. In the transverse section of A. orientalis, the stem of this species was quadrangular with well-defined collenchyma in the four angles. It was also determined that there were 2-3 layers of sclerenchyma in the outer side of phloem in this study. Quadrangular stem with well-defined collenchyma in th e four angles and scleranchymatous tissue surrounds the phloem groups of vascular bundles were observed in other members of Lamiaceae family (Metcalfe and Chalk, 1972; Kandemir, 2003; Baran and Ãâ€"zdemir, 2006; Kahraman et al., 2010). The vascular cambium located between the phloem and the xylem was 1-2 rowed layer in the stem of A. orientalis. The vascular cambium was seen in the cross-sections of other members of Lamiaceae family (Dinà § and Ãâ€"ztà ¼rk, 2008; Baran and Ãâ€"zdemir, 2009). There were big vascular bundles on the corners of A. orientalis stem whereas a lot of small bundles between the corners were observed in the present study. There were also starch particles in the paranchymatous cortex cells of stem. The leaf of A. orientalis had 2-3 layered palisade parenchyma and 2-3 layered spongy parenchyma cells. Baran and Ãâ€"zdemir (2009) also stated that Lamium lycium which is the member of Lamiaceae family has 2-3 layered palisade parenchyma and 2-3 layered spongy parenchyma cells as well. These results were parallel to our results mentioned above. On the other hand, the stoma type of A. orientalis was diasitic and the leaf was bifacial. According to Metcalfe and Chalk (1972), diasitic stoma type was most common in Lamiaceae family. Diasitic stoma and bifacial mesophyll type were also observed on the leaf of Ajuga chamaepitys and Ajuga reptans (Akà §in et al., 2006). The stomata were observed on both upper and lower surfaces of the leaf. Metcalfe and Chalk (1972) stated that the structure of the vascular bundles in the petiole of the species in the Lamiaceae could be used as a diagnostic character. The structure of petiole shows differences between genera and species. In addition to this, useful petiole anatomical characters are determined in designated taxonomical structures of some species (Shaheen, 2007; Eric et al., 2007). In the petiole of A. orientalis, there was one big vascular bundle in the center and 4-5 small vascular bundles at e ach corner of the petiole. Akà §in et al. (2011) found that Ajuga reptans has a total of nine vascular bundles: one big bundle in the middle and 4 vascular bundles at each corner. The vascular bundles of leaf were surrounded by sclerenchymatic cells. Collenchyma in the petiole is 2-3 layered at the each corners. In the cross-section of calyx and corolla, both adaxial and abaxial epidermis cells were covered with cuticle. In addition to this, there were a vascular bundle in the midrib of calyx and corolla. The adaxial epidermis cells of corolla were papillose type. The most distinguishing characteristics of the species in the anatomical structure were the presence of glandular hairs on the surface layers on stem, leaves, petiole, calyx and corolla. Like other other members of the Lamiaceae, A. orientalis had both glandular as well as non-glandular trichomes. Glandular trichomes were mainly observed on calyx and corolla, but non-glandular ones were found on the stem, leaf surface and petiole. According to Metcalfe and Chalk (1972), having glandular and non-glandular trichomes are important anatomical characters. 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Structure of glandular hairs and identification of the main components of their secreted material in some species of the Labiatae. Israel J. Bot. 34: 31-45. 1 [a1]Should add faimly of this plant which is â€Å"Lamiaceae† [a2]Should add stem to be root, stem [a3]Remove â€Å"medicinal taxon† [a4]Should mention pith shape in the stem [a5]Better to be other world†detected† [a6]Should be â€Å"diastatic†